Dissipative Organization of DNA Oligomers for Transient CatalyticFunction

Read the full article here: Angew. Chem.Int. Ed.2022,e202113477

by: Deng, J., Liu, W., Sun, M., Walther, A. “Dissipative Organization of DNA Oligomers for Transient Catalytic Function” Angew. Chem. Int. Ed. e202113477 (2022)

The development of synthetic non-equilibrium systems opens doors for man-made life-like materials. Yet, creating distinct transient functions from artificial fuel-driven structures remains a challenge. Building on our ATP-driven dynamic covalent DNA assembly in an enzymatic reaction network of concurrent ATP-powered ligation and restriction, we introduce ATP-fueled transient organization of functional subunits for various functions. The programmability of the ligation/restriction site allows to precisely organize multiple sticky-end-encoded oligo segments into double-stranded (ds) DNA complexes. We demonstrate principles of ATP-driven organization into sequence-defined oligomers by sensing barcode-embedded targets with different defects. Furthermore, ATP-fueled DNAzymes for substrate cleavage are achieved by transiently ligating two DNAzyme subunits into a dsDNA complex, rendering ATP-fueled transient catalytic function.